How To Do A Saliva Adrenal Test

Important update on Adrenal Testing

We now recommend the DUTCH Hormone Test instead of the saliva test for greater accuracy and more detailed information about the adrenal and sex hormones.

But if you are still wanting to know how to do a saliva adrenal test read on.

Click Here to find out more about the DUTCH Hormone Test

Adrenal Fatigue can have many symptoms and cannot be diagnosed very accurately from traditional blood tests. There are different stages of Adrenal Fatigue and to get an accurate diagnosis it is necessary to do a DUTCH Hormone Test, this measures DHEA and cortisol at 4 different times during the day.

If you already have your test kit these instructions will help you to understand how to do the test correctly, if you would like to order an adrenal stress test just click here 


If you are uncertain of the collection procedure after reading these instructions, please contact our Customer Service Staff on 1300 688 522 who will clearly explain the procedure or email me at

Before proceeding, please check through the following guidelines. Without taking these precautions your results may be inaccurate.

  •  Please follow the collection instructions accurately.
  •  On the day of collection; AVOID caffeine, alcohol and nicotine (don’t smoke two hours before test).
  •  During the 2 hours prior to collection; DO NOT eat, brush or floss your teeth or use mouthwash. You may drink water during this time.
  •  Before beginning collection; WASH hands thoroughly with soap and water.
  • Each sample can take 5-20 minutes to collect. Stay hydrated throughout the day to help with saliva production, and tips to increase saliva flow include running your tongue around your cheeks, yawning and the smell of food.


1. Please collect saliva samples for each of the tubes provided at the times of day indicated below. Generally, if only one tube is provided you need to collect the sample between 6-8 am, and this is only for testing sex hormones.

how to do a saliva adrenal test

Fill test tube to black line

To collect the sample, rinse your mouth with a little water and wait 5 minutes before beginning to collect saliva in the tube. During the collection, do not cough, clear your throat or collect mucous from the back of your throat into the collection tube. Allow saliva to accumulate naturally in your mouth, and when an adequate amount is collected, transfer this to the saliva collection tube.
3. FILL THE COLLECTION TUBE to the line with liquid saliva. Avoid foamy saliva or ‘bubbles’. Tap the tube with the fingernail or tap on a hard surface to reduce bubbles. Insufficient saliva samples cannot be processed. ENSURE THE CAP IS SCREWED ON CORRECTLY AND TIGHTLY TO AVOID LEAKAGE IN TRANSIT.
4. Make sure collection time and date are written on the tube and the cap is replaced tightly to avoid leakage. Place the filled tube into the refrigerator until shipping to the lab. DO NOT FREEZE.
5. Repeat steps 1 – 4 for each collection tube at the following times times 6-8am ( ideally within 30 minutes of waking up ), Midday, 4pm and 8-10pm or 30 minutes before going to bed. Ensure that you label all of the tubes of saliva correctly with the time collected, including am and pm
If doing a Female Hormone Profile (Basic or Extensive) please collect the
sample on day 19-21 of your cycle.
6. Fill out patient details including name, address, date of birth, contact number on the referral form.

Make sure the referral form is filled out and is returned with the sample

Make sure the referral form is filled out and is returned with the sample

7.Place all paperwork and tubes into Specimen Transport Bag then into padded pre-paid express post bag and post to NutriPATH. The results take 1-2 weeks to process.

Do the test on a Sunday, Monday or Tuesday, so that the sample does not have to travel over the weekend.

Order An Adrenal Test

To find out more about ordering a saliva adrenal test go to Saliva Hormone Test Order Page or if you would like to do the most comprehensive adrenal and sex hormone test available check out the DUTCH Hormone Test

About the Author Michael

Michael is head consultant at Planet Naturopath - Functional Medicine and Nutrition Solutions. He is a registered naturopath with the Australian Natural Therapists Association (ANTA) and works with clients from all over the world via video or phone consultations. He is a degree qualified naturopath from the Endeavour College of Natural Medicine in Australia with 18 years of experience. He uses advanced testing methods, nutritional medicine, herbal medicine, and lifestyle advice to help you stay healthy. He is a Kalish Method-trained practitioner that keeps updating his education with Chris Kresser. Michael completed Dr. Terry Wahls practitioner training program, a 12-month program with ongoing training that helps understand the underlying cause and treatment of MS and autoimmune conditions. He keeps up to date with the latest research into health and natural medicine through the Metabolic Fitness Pro course with Dr. Bryan Walsh.

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Leave a Comment:

5 Common Causes of Digestion Problems - Paleo FoodiesPaleo Foodies says September 29, 2014

[…] adrenal saliva stress test can help to identify how well your body is responding to stress and help to determine a clear […]

karine says June 22, 2017

Bonjour, je fais le test de salive Cortisol et Dhea. J’ai 33 ans et je prends la pilule anticonceptionnelle en continue. Est-ce que je peux faire le test n’importe quand? Et je prends aussi de la synthroid le matin, est-ce que je dois prendre le test de salive avant ou après? Merci!

    Michael says July 9, 2017

    I am afraid my french is limited to Bonjour and Merci

Geri Koslowsky says July 27, 2017

How long can a saliva stay in tube before testing

    Michael says July 27, 2017

    Ideally you want to get the sample back to the lab asap, but the samples will still be fine after 5-7 days. Saliva testing is not as accurate and the samples are less stable that the DUTCH hormone test

Fritha says July 29, 2017

How much saliva is sufficient for the test? I have sjogrens syndrome and at the most I can only fill a 5m tube with 1 to 2 mls with liquid saliva, then only bubbles.

Biohealth says January 28, 2019

Thanks for sharing such a piece of nice information about Adrenal saliva. Adrenal saliva test or adrenal insufficiency test is an efficient test for the diagnosis of Addison’s disease. The adrenal saliva test is also known by some other names including adrenal fatigue saliva test and adrenal fatigue test. This test measures the level of minerals and hormone in the body. Magnetic Resonance Imaging (MRI) or Computerized Tomography Scan (CT Scan) is used to see the size of adrenal glands of the patient.

Biohealth Laboratory says February 25, 2019

Hormone saliva test also checks the level of supplemented hormones with their uses and absorption. According to a study, the measurements of salivary progesterone confirm applies progesterone is absorbed, regardless of lack of serum progesterone change. Therefore, this test not only can determine if bioidentical hormone therapy is absorbed but influence supplementation dosage as well.

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